Fabrication of Gelatin Modified Microfibrous Paper as Scaffold for Tissue Engineering Application
Cotton is cheap and easily available and it has been widely used as bandage material because of its higher degree of biocompatibility and non-cytotoxic nature. Cotton can be a good raw material to design scaffold for tissue engineering applications because of its fibrous structure, which can accelerate cell adhesion, growth, migration, proliferation and differentiation of the cells. Cotton cellulosic microfibrous based paper scaffold can be easily surface modified by using different polymers to alter its biodegradability rate and cell attachment. In this work, we successfully fabricated paper scaffolds of different thickness using British sheet former machine. We also reported the use of fabricated scaffold modified with gelatin to make biocompatible for the sustained growth of mouse macrophage cell in scaffold. Microfibers based scaffold could also overcome the limitations of nanofibrous material based scaffold for tissue engineering application. Thus cotton based paper scaffolds would be a promising candidate for various tissue regeneration applications.
Physico-chemical and mechanical properties of scaffolds were tested using FT-IR spectroscopy, Scanning electron microscopy, H.Tensile (Frank-PTI Gmbh). Further porosity was determined by Gurley densometer. Thus in this study paper scaffold of various thickness ranges (60μm-300μm) with respective tensile strength range (12MPa-22.7MPa) were fabricated. To the best of our knowledge, this is the first study reporting the fabrication of scaffold by paper making process using surgical cotton that is biocompatible and non-toxic. So this study suggests that fabricated scaffold have huge prospective as a bioactive, well-designed and economical scaffold stand for tissue engineering.
Keywords - Cellulose, Cotton, Scaffold and Tissue Engineering