Paper Title
Preparation and Evaluation of Poly Herbal Gel Containing Extract of Aegle Marmelos, Calendula Officinalis, Fenugreek as an Antimicrobial Agent

Abstract
Background: -Ayurveda is most traditional and oldest Indian healing systems. The beliefs behind Ayurveda are that it has no side effects prevent unessential problems and provide a healthy and long life. Ayurveda means the use of natural resources to remove the main cause of disease. Herbal remedies have been around the world for a long time and have been used in ancient Chinese, Greek, Egyptian, and Indian medicine for various medicinal purposes. Aegle Marmelos (Bael) leaf extract is taken twice daily to treat ophthalmia, ulcers, and intestinal worms, among other ailments. Poultice made from bael leaf is used in the treatment of eye conditions. Leaf juice has a variety of therapeutic applications, with the most notable being the treatment of diabetes. Fenugreek is used to cure red spots around the eyes, as well as to soften the throat and chest and to give relief from coughing. To the use of this plant in the form of infusion, powder, pomade, and decoction have been extremely popular in Iranian traditional medicine. The plant may be used to wash one's vaginal linings. This plant is used as an emollient in the lack of appetite, treatment of pellagra, and gastrointestinal problems, as well as a general tonic Calendula officinalis leaves are used to treat varicose veins on the outside of the body by infusing them. In Europe, the leaves are diaphoretic and resolvent in nature, while the blooms are employed as an emmenagogue, and antispasmodic stimulant in the Canada and United States. The flowers were decocted and served as a posset drink when smallpox and measles were common in England, and the fresh juice was used to treat jaundice There is a various type of plants that have medicinal property and they are widely used in the treatment of diseases related to skin. They are also used because of their antibacterial activity. The use of gel on the affected areas offers significant benefits in the immediate release of the drug directly into the affected site when compared to ointement and cream. Today, the “rejuvenation” of Ayurvedic Poly Herbal Formulations has occurred worldwide, due to their comparative effectiveness, fewer side effects and better acceptance than allopathic medicines. Objective: -This study is done for the preparation and evaluation of poly herbel gel Containing extract of the leaves ofAegle Marmelos, Calendula Officinalis, and Fenugreek. Materials and Methods: -In the present study we prepared gel formulations (formulations 1 to 5) which comprised of extract of the leaves ofAegle Marmelos, Calendula Officinalis, and Fenugreek. The base was prepared by using carbapol 940, propylene glycol- 400, ethanol, methyl paraben, propylparaben, EDTA, triethanolamine and required amount of water in a quantity sufficient to prepare 25 g. The prepared formulations were screened for their antimicrobial activity by agar well diffusion technique against S. aureus. The formulations were also evaluated for appearance, pH, viscosity, spreadability, extrudability. Result: -The results of the studies revealed that all formulation from F1 – F5 under study showed better zone of inhibition as compared with the base. However, formulation F2 exhibited maximum activity against the selected strains which may be attributed to its greater amount of herbal extracts as compared to other formulation. Conclusion: -In this study we have found that these formulations Showes antimicrobial activity and can be used safely on human skin. Keyword - AegleMarmelos, Calendula Officinalis, Fenugreek, Poly herbal Gel.